135 - DEFB1 c.-20G>A Variant in Mexican Patients with Early Childhood Caries

Purpose: To analyze the association of DEFB1 c.-20G >A variant with early childhood caries in Mexican patients.

 Materials and

Methods: Seventy-seven children with early childhood caries (ECC) were included, previous informed parental consent. Clinical examination was realized according to the ICDAS-II (International Caries Detection and Assessment System) protocol. DNA was extracted from buccal mucosa cells. The c.-20G >A variant was identified by PCR-RFLP´s method. The association was estimated by odds ratio (OR) test and 95% of confidence Interval (CI).

Results: The age range of the patients was 6-71 months old. The patients in this study were separated in two groups according to ICDAS-II, the group I included 1-2 code (injury only in enamel) and was integrated by 13 ECC patients, while the group II included 3-6 code (injury found also in dentine) and were 64 patients. The DEFB1 AA genotype was more frequent in group II than I (39% and 15%, respectively). The comparison between both groups showed the AA genotype is associated with group II (ICDAS-II code 3-6) (OR=9.38, CI=1.14-74.84 and P=.02). Moreover, the DEFB1 A allele was also more frequent in group II than I (66% and 46% respectively) However, no significant differences were found for the A allele although the P value was in borderline (OR=2.31, CI=0 .99-5.42 and P=.05).

 

Conclusion: The AA genotype of DEFB1 c.-20G >A variant is a risk factor for early childhood caries development in Mexican patients.

Identify Supporting Agency and Grant Number: Research supported by Universidad de Guadalajara.