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Caries

250 - Remineralization Effects of Salivary Vitamin D on Primary Dentition Caries

Friday, May 24, 2024
1:45 PM – 1:55 PM EST
Location: Exhibit Hall (back left corner of the hall, space 137)

    Presenting Author(s)

  • Noor M. Al Shaltoni, DDS

    Resident
    Boston University, Boston, MA
    Boston, Massachusetts, United States

    • Research Mentor(s)

  • yF

    yuwei Fan, PhD

    Boston University
    boston, Massachusetts, United States

    • Program Director(s)

  • KD

    Keri Discepolo, DDS, MPH

    Post Graduate Program Director of Pediatric Dentistry
    Boston University Henry M. Goldman School of Dental Medicine
    Boston, Massachusetts, United States



Purpose: The aim of this in-vitro study is to investigate the remineralization effect of salivary vitamin D on artificially initiated carious lesions by analyzing changes in enamel microhardness and crystallization.



Methods: Thirty-one unidentified extracted human primary teeth were utilized for the purpose of this in-vitro study. Teeth were divided into 4 groups (n=8 teeth in groups A, C, D, n=7 teeth in group B) based on the concentration of vitamin D as follows; 1. artificial saliva without vitamin D as a control group, 2. artificial saliva with 20 nM of vitamin D to indicate deficiency, 3. artificial saliva with 40 nM of vitamin D to indicate insufficiency, and 4. artificial saliva with 100 nM of vitamin D to indicate sufficiency of vitamin D. Vickers microhardness testing (HV) was conducted before the initiation of artificial carious lesions to record the baseline. Subsequently, HV was recorded at 4 phases (n=7 teeth per group): at baseline, following immersion in artificial carious solution, midway through treatment cycling, and at the end of the 10-day experiment. Additionally, crystal morphology was examined using a field emission scanning electron microscope (FESEM) with different magnifications (n=1 tooth per group). Descriptive analysis and multiple factorial ANOVA used for data analysis. field emission scanning electron microscope (FESEM) with different magnifications (n=2 teeth per group).



Results: The mean values of enamel surface microhardness exhibited a noticeable increase in groups C and D) compared to the group A and group B. Moreover, there is a statistically significant difference in enamel surface microhardness among groups B, C, D and control group A (p < 0.05). Additionally, examination under the FESEM revealed the presence of plate-like crystals on the enamel surface of teeth in groups A and C, whereas enamel surfaces treated with 100 nM VD3 (group D) displayed needle-like apatite crystallites. 





Conclusion:  The application of Vitamin D demonstrated a notable enhancement in the densely crystallized enamel morphology and an improvement in surface microhardness. These findings emphasize the clinical significance of maintaining optimal concentrations of vitamin D in children for the effective remineralization of incipient carious lesions.



 


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