245 - Chitosan Based Sponges for Prospective Hemostatic Applications

Purpose: The aim of this study was to determine the physicochemical and biological properties of chitosan sponges for to be used as a hemostatic material in dental treatments.

Methods: 200 mg of low molecular weight chitosan, 10 mg of lysine and 52 μl polyethylenimine with 4% acetic acid were used, which were lyophilized to obtain chitosan (Ch), chitosan-lysine (Ch-K) and chitosan-polyethylenimine (Ch-PEI) sponges and to vary their chemical and biological properties. Physicochemical characterizations were performed by thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Biological properties were determined by cell viability and coagulation with the Lee-White method.

Results: The presence of C, N and O elements was detected by XPS. FTIR confirmed the crosslinking of chitosan with K and PEI. The decomposition temperature (Td) of the foams ranged from 272° to 283°C. By SEM, porous morphology was observed in the samples, which was favorable for cell growth. Cell viability was analyzed by ANOVA test (p < 0.001), which yielded a statistically significant value. Coagulation was also statistically evaluated using an ANOVA test, and the result was not statistically significant (p=0.062).

Conclusions: Ch, Ch-K, Ch-PEI sponges have physicochemical and biological qualities necessary to be used as hemostatic materials in dentistry. From the study materials, the chitosan sponge showed better hemostatic and cell viability properties.

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This research project was supported by Autonomous University of Yucatan (UADY)