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Preventive

522 - Analysis of Probiotic Candidates’ Suppression of Candida albicans Mediated Inflammation

Saturday, May 25, 2024
1:30 PM – 1:40 PM EST
Location: Exhibit Hall (back left corner of the hall, space 137)

    Presenting Author(s)

  • SA

    Samuel L. Allen, BS

    Dental Student
    University of Iowa, Iowa City, IA
    University of Iowa College of Dentistry
    Coralville, Iowa, United States

    • Co-Author(s)

  • MZ

    Min Zhu, MS

    University of Iowa

    • Research Mentor(s)

  • JB

    Jeffrey A. Banas, PhD

    Director, Iowa Institute for Oral Health Research
    University of Iowa College of Dentistry, Iowa City, IA
    Iowa City, Iowa, United States

    • Program Director(s)

  • SB

    Sheila Britton, n/a

    SRG Program Coordinator
    University of Iowa, Iowa City, Iowa
    Iowa City, Iowa, United States

Abstract:



Purpose: Candida albicans has been linked to oral diseases ranging from dental caries to oral cancer. It appears to enhance the cariogenicity of the dental pathogen Streptococcus mutans, and it’s also associated with pro-inflammatory changes in the oral microbiota. The goal of this project was to test probiotic candidates, previously isolated from children with a caries-free history and partially screened for properties related to caries prevention, for inhibition of C. albicans and suppression of inflammation.



Methods: C. albicans was grown in the presence of probiotic candidates in addition to health and disease associated controls to evaluate inhibition of C. albicans and to determine their influence on hyphae formation. Candidates were assayed using quantitative polymerase chain reaction (QPCR) to quantify relative expression of potential oncogenesis-related inflammatory cytokines, transcription factors/genes and enzymes in co-cultures with two squamous cell carcinoma cell lines and epithelial cell models.



Results: None of the candidate strains tested had the ability to inhibit the growth of C. albicans; however, four of nine candidates displayed the ability to reduce hyphae formation or coexist with Candida without inducing increased hyphae formation indicating minimal or reduced stress response from C. albicans. QPCR revealed two candidates, A2 and A3, significantly decreased expression of BCL3, BIRC3, JUN, IFIT3, SERPINE1, MMP-1, IL-6 and IL-8 compared to cultures of C. albicans alone.



Conclusion: Certain bacterial strains from children with a caries free dental health history have the potential to suppress chronic inflammation which may aid in the prevention of oral disease associated with C. albicans.

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