244 - Antimicrobial Effect of Pastes Used in Necrotic Primary Molars Therapy

Purpose: The objective of this in vitro study is to evaluate, the antimicrobial effect of two pastes used in the treatment of molar necrotic Pulp therapy.

Methods: The Kirby Bauer test was used to evaluate the antimicrobial effect of two pastes; Ultrapex and one composed of tetracycline, chloramphenicol, zinc oxide and eugenol (CTZ) against bacterial strains present in necrotic pulps, including; Enterococcus faecalis, Fusubacterium nucleatum, Porphyromona gingivalis and Prevotella intermedia. A negative and positive control was used clorhexidine and bacterial medium, respectively. Under anaerobiosis criteria and after 72 hours of incubation, the inhibition halos were measured.

Results: The average of the measurements obtained were 57.75 millimeters for the plates with CTZ and 17.5 millimeters for the plates with clorhexidine. Enterococcus faecalis was the strain with the highest resistance. The Vitapex paste did not form inhibition halos.

Conclusions: From the analysis of the results it can be affirmed that the CTZ paste presented high values of bacterial inhibition against bacteria prevalent in necrotic pulps while the Ultrapex paste didn´t show antimicrobial effects against studied strains.

Identify Supporting Agency and Grant Number: Research supported by Universidad Autonoma de Yucatan